Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

Marek Plata, William Hale, Manvendra Sharma, and Marcel Utz

Lab on a Chip, 2021

We present a microfluidic platform that allows in operando nuclear magnetic resonance (NMR) observation of serial mixing experiments. Gradually adding one reagent to another is a fundamental experimental modality, widely used to quantify equilibrium constants, for titrations, and in chemical kinetics studies. NMR provides a non-invasive means to quantify concentrations and to follow structural changes at the molecular level as a function of exchanged volume. Using active pneumatic valving on the microfluidic device directly inside an NMR spectrometer equipped with a transmission-line NMR microprobe, the system allows injection of aliquots and in situ mixing in a sample volume of less than 10 μL.

Graphical abstract: Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

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